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Imagem de célula única de cérebro inteiro e análise de cérebros de camundongos neonatais intactos usando ressonância magnética, limpeza de tecidos e microscopia de folha de luz
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Imagem de célula única de cérebro inteiro e análise de cérebros de camundongos neonatais intactos usando ressonância magnética, limpeza de tecidos e microscopia de folha de luz
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08:49 min
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August 01, 2022
DOI:
1UNC Neuroscience Center,University of North Carolina, Chapel Hill, 2Department of Genetics,University of North Carolina, Chapel Hill, 3Department of Psychiatry,University of North Carolina, Chapel Hill, 4Center for Animal Magnetic Resonance Imaging,The University of North Carolina at Chapel Hill, 5Biomedical Research Imaging Center,The University of North Carolina at Chapel Hill, 6Department of Neurology,The University of North Carolina at Chapel Hill, 7Department of Computer Science,The University of North Carolina at Greensboro
Summary
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Este protocolo descreve métodos para a realização de ressonância magnética, limpeza e imunomarcação de cérebros de camundongos intactos usando iDISCO+, seguido por uma descrição detalhada de imagens usando microscopia de folha de luz e análises a jusante usando NuMorph.
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Whole-brainSingle-cellImagingAnalysisIntactNeonatalMouseBrainMRILight-sheet MicroscopyImage AnalysisNuclei QuantificationComputational PipelinePreprocessingCell DetectionImaging TimeComputational ResourcesSample MountingSample OrientationZoom MagnificationLight SheetNumerical ApertureDynamic FocusingLaser PowerLight Sheet WidthTile OverlapCondaNuMorphMATLABImage ProcessingIntensity Adjustment
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Cite this Article
Kyere, F. A., Curtin, I., Krupa, O., McCormick, C. M., Dere, M., Khan, S., Kim, M., Wang, T. W., He, Q., Wu, G., Shih, Y. I., Stein, J. L. Whole-Brain Single-Cell Imaging and Analysis of Intact Neonatal Mouse Brains Using MRI, Tissue Clearing, and Light-Sheet Microscopy. J. Vis. Exp. (186), e64096, doi:10.3791/64096 (2022).
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