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Investigando a patogênese da mutação MYH7 Gly823Glu em cardiomiopatia hipertrófica familiar usando um modelo de camundongo
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03:45 min
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August 08, 2022
DOI:
1Department of Cardiovascular Surgery, Guangdong Provincial Hospital of Traditional Chinese Medicine,the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 2The Second Clinical College of Guangzhou University of Chinese Medicine, 3Department of Cardiovascular Surgery, The First Affiliated Hospital,Jinan University, 4Guangdong Provincial Key Laboratory of Laboratory Animals,Guangdong Laboratory Animals Monitoring Institute, 5Department of Anesthesiology, Guangdong Provincial Hospital of Chinese Medicine,the Second Affiliated Hospital of Guangzhou University of Chinese Medicine
Summary
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Com base na família de cardiomiopatia hereditária familiar encontrada em nosso trabalho clínico, criamos um modelo de camundongo C57BL / 6N com uma mutação pontual (G823E) no locus MYH7 do camundongo através da engenharia genômica mediada por CRISPR / Cas9 para verificar essa mutação.
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Cite this Article
Xia, Y., Hu, J., Li, X., Zheng, S., Wang, G., Tan, S., Zou, Z., Ling, Q., Yang, F., Fan, X. Investigating the Pathogenesis of MYH7 Mutation Gly823Glu in Familial Hypertrophic Cardiomyopathy using a Mouse Model. J. Vis. Exp. (186), e63949, doi:10.3791/63949 (2022).
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