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발달 중인 (개구리) 배아의 세포 계통 유도 질량 분석법 단백질체학
Journal JoVE
Chimie
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Journal JoVE Chimie
Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing (Frog) Embryo
DOI:

09:18 min

April 21, 2022

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Chapitres

  • 00:04Introduction
  • 00:52Identification and Isolation of Single Cells
  • 01:27Labeling Cells of Interest
  • 02:42Isolation of Labeled Clones
  • 03:47Proteomic Analysis
  • 05:39Separation by Capillary Electrophoresis
  • 06:37Separation by Liquid Chromatography
  • 07:12Ionization and Detection of Peptides
  • 07:30Results: Characterization of Protein Expression in Single Cells and Cell Lineages of Xenopus laevis Embryos
  • 08:25Conclusion

Summary

Traduction automatique

여기에서 우리는 척추동물 Xenopus laevis 배아에서 알려진 조직 운명을 가진 세포 계통의 질량 분석 기반 단백질체 특성 분석을 설명합니다.

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