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ヒト人工多能性幹細胞における複数のミトコンドリアパラメータとその神経およびグリア誘導体のフローサイトメトリー解析

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Neurosciences

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Journal JoVE Neurosciences

Flow Cytometric Analysis of Multiple Mitochondrial Parameters in Human Induced Pluripotent Stem Cells and Their Neural and Glial Derivatives

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ヒト人工多能性幹細胞における複数のミトコンドリアパラメータとその神経およびグリア誘導体のフローサイトメトリー解析

3,479 Views

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06:09 min

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November 08, 2021

DOI:

10.3791/63116-v

DOI:

10.3791/63116-v

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06:09 min

November 08, 2021

•

4 Views

Kristina Xiao Liang*^1,2, Anbin Chen*^1,2,3,4, Cecilie Katrin Kristiansen², Laurence A. Bindoff²

¹Department of Clinical Medicine (K1),University of Bergen, ²Neuro-SysMed, Center of Excellence for Clinical Research in Neurological Diseases,Haukeland University Hospital, ³Department of Neurosurgery, Qilu Hospital and Institute of Brain and Brain-Inspired Science, Cheeloo College of Medicine,Shandong University, ⁴Shandong Key Laboratory of Brain Function Remodeling

Tags

Flow Cytometry Mitochondrial Parameters Human Induced Pluripotent Stem Cells Neural Derivatives Glial Derivatives FCCP TMRE MTG Mito Sox Cell Dissociation Paraformaldehyde Methanol Blocking Buffer

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