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免疫肽组学：分离小鼠和人MHC I类和II类相关肽用于质谱分析

Journal JoVE
Biochimie

This content is Free Access.

Journal JoVE Biochimie

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

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免疫肽组学：分离小鼠和人MHC I类和II类相关肽用于质谱分析

8,927 Views

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09:32 min

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October 15, 2021

DOI:

10.3791/63052-v

DOI:

10.3791/63052-v

•

09:32 min

October 15, 2021

•

77 Views

Isabelle Sirois*¹, Maxim Isabelle*¹, Jérôme D. Duquette¹, Frederic Saab¹, Etienne Caron^1,2

¹CHU Sainte-Justine Research Center, ²Department of Pathology and Cellular Biology, Faculty of Medicine,Université de Montréal

Summary

在这里，我们提出了一种从小鼠和人细胞系纯化MHC I类和II类肽复合物的方案，提供高质量的免疫肽组学数据。该协议侧重于使用市售抗体进行样品制备。

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