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Ein dreidimensionales Sphäroidmodell zur Untersuchung der Tumor-Stroma-Wechselwirkung bei hepatozellulären Karzinomen
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1Department of Biochemistry, Faculty of Pharmacy,Minia University, 2National Institute for Health Research Birmingham Liver Biomedical Research Unit and Centre for Liver and Gastrointestinal Research, Institute of Immunology and Immunotherapy,University of Birmingham, 3Newcastle Fibrosis Research Group, Biosciences Institute, Faculty of Medical Sciences,Newcastle University, 4Newcastle University Translational and Clinical Research Institute, Faculty of Medical Sciences,Newcastle University, 5The Liver Unit, Department of Medicine, Freeman Hospital,Newcastle-upon-Tyne Hospitals NHS Foundation Trust
Chapitres
- 00:05Introduction
- 01:20Cell Preparation
- 02:17Cell Collection and Counting
- 04:14Collection of Fibroblast Conditioned Media
- 05:17Heterotypic Tumor/Stromal Spheroids
- 06:29Homotypic Hep3B Spheroids in LX2 Conditioned Media
- 08:18Calculation of Spheroid Volume
- 09:17Results: In Vitro Creation and Culture of 3D Tumor Spheroid Model
- 11:28Conclusion
Es fehlen umfassende In-vitro-Modelle , die die relevante menschliche Krankheit getreu rekapitulieren. Die aktuelle Studie präsentiert die dreidimensionale (3D) Tumorsphäroidbildung und -kultur, ein zuverlässiges In-vitro-Werkzeug zur Untersuchung der Tumor-Stroma-Interaktion beim menschlichen hepatozellulären Karzinom.
