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Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line
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Immunologie et infection
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Journal JoVE Immunologie et infection
Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line

Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line

DOI:
10.3791/62652-v

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06:38 min

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August 02, 2021

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, , , ,
1Price Institute of Surgical Research, Department of Surgery,University of Louisville

Chapitres

  • 00:05Introduction
  • 00:56Culturing and Maintenance of THP‐1 Monocyte-like Cells
  • 02:59Seeding of THP‐1 cells and Differentiation into M0 Macrophages
  • 04:01Polarization of M0 Macrophages into M2‐like Macrophages
  • 04:39Detaching and Harvesting Macrophages for Flow Cytometry
  • 05:14Results: Macrophage Differentiation and Polarization into an M2‐Like Phenotype
  • 05:56Conclusion

Summary

Traduction automatique

Traduction automatique

M2-like tumor-associated macrophages (TAM) are associated with tumor progression and poor prognosis in cancer. This protocol serves as a detailed guide to reproducibly differentiate and polarize THP-1 monocyte-like cells into M2-like macrophages within 14 days. This model is the basis to investigate the anti-inflammatory effects of TAM within the tumor microenvironment.

Tags

Keywords: MacrophageDifferentiationPolarizationM2-like PhenotypeTHP-1 CellsAnti-inflammatoryTumor-associated MacrophagesColon CancerCell CultureCell CountingCell Viability

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