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Chapitres
- 00:04Introduction
- 00:41Preparation of Male and Female Mosquitoes
- 01:11Preparation of 0.2% RhB Sucrose Solution and Feeding of Male Mosquitoes
- 01:52Checking for RhB Fluorescence in Male Mosquitoes
- 02:46Mating Competitiveness Assay
- 05:36Results: Assessment of Mating Competitiveness in Male Mosquitoes
- 06:27Conclusion
Here, we present a protocol for studying mating competitiveness of male Aedes aegypti using fluorescent dye as a marker. Female mosquitoes are exposed to both marked and unmarked males for copulation. Post mating, their spermathecae are examined under a fluorescence microscope to determine their mating partner.
