Journal
/
Immunologie et infection
/
Knock-in de genes por CRISPR/Cas9 y clasificación celular en líneas de macrófagos y células T
Journal JoVE
Immunologie et infection
Author Produced
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Immunologie et infection
Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines
Please note that all translations are automatically generated. Click here for the English version.

Knock-in de genes por CRISPR/Cas9 y clasificación celular en líneas de macrófagos y células T

9,722 Views

•

11:32 min

•

November 13, 2021

DOI:

10.3791/62328-v

DOI:
10.3791/62328-v

•

11:32 min
November 13, 2021

•

9 Views
, , , , , , , , ,
1The Laboratory of Genetic Regulators in the Immune System, School of Laboratory Medicine,Xinxiang Medical University, 2Centre d’Immunologie de Marseille-Luminy,Aix Marseille Université

Tags

CRISPR/Cas9Gene Knock-inMacrophageT Cell LinesROSA26 LocusSgRNA DesignHomologous RecombinationTargeting VectorFluorescent ReporterCell Sorting

Read Article

Cite this Article

Zhang, L., Huang, R., Lu, L., Fu, R., Guo, G., Gu, Y., Liu, Z., He, L., Malissen, M., Liang, Y. Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines. J. Vis. Exp. (177), e62328, doi:10.3791/62328 (2021).

Download .ris file

Copy

Share Video

.

Copy