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Monitoramento de Elastase e Cathepsin G Atividade em Amostras de Espiuma Humana
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Monitoramento de Elastase e Cathepsin G Atividade em Amostras de Espiuma Humana
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09:23 min
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May 21, 2021
DOI:
1Translational Lung Research Center Heidelberg (TLRC),German Center for Lung Research (DZL), 2Dept. of Translational Pulmonology,University of Heidelberg, 3Molecular Medicine Partnership Unit (MMPU), European Molecular Biology Laboratory (EMBL),University of Heidelberg, 4Faculty of Biosciences, Collaboration for Joint Ph.D. Degree between EMBL and Heidelberg University,University of Heidelberg, 5Dept. of Pediatric Pulmonology, Immunology and Critical Care Medicine,Charité – Universitätsmedizin Berlin, 6Berlin Institute of Health (BIH), 7German Center for Lung Research (DZL), Associated Partner Site, Berlin, 8Dept. of Chemical Physiology and Biochemistry,Oregon Health and Science University
Summary
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Os protocolos aqui descritos fornecem um guia para visualizar e quantificar a atividade de proteases neutrófilos na escarro humana. As aplicações dessa análise abrangem desde a avaliação de tratamentos anti-inflamatórios, até validação de biomarcadores, triagem de medicamentos e grandes estudos clínicos de coorte.
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Frey, D. L., Guerra, M., Mall, M. A., Schultz, C. Monitoring Neutrophil Elastase and Cathepsin G Activity in Human Sputum Samples. J. Vis. Exp. (171), e62193, doi:10.3791/62193 (2021).
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