Visualizing the Effects of Oxidative Damage on Drosophila Egg Chambers using Live Imaging

Journal

Biologie du développement

Journal JoVE
Biologie du développement
Author Produced

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Journal JoVE Biologie du développement

Visualizing the Effects of Oxidative Damage on Drosophila Egg Chambers using Live Imaging

DOI:

10.3791/62157-v

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09:20 min

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April 10, 2021

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Kelsey M. Sheard, Rachel T. Cox

¹Department of Biochemistry and Molecular Biology,Uniformed Services University

Chapitres

00:00Introduction
01:14Preparing Single Ovarioles for Live Imaging
05:34H₂O₂ Treatment and Live Imaging
06:37Results: Representative Changes in Mitochondrial and Clu Bliss Particle Subcellular Localization After H₂O₂ Addition
08:25Conclusion

Summary

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The objective of this protocol is to use live imaging to visualize the effects of oxidative damage on the localization and dynamics of subcellular structures in Drosophila ovaries.

Visualizing the Effects of Oxidative Damage on Drosophila Egg Chambers using Live Imaging

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Chapitres

Summary

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