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ワクチン接種マウスにおける抗原特異的CD8 T細胞の細胞周期解析のためのDNA/Ki67ベースのフローサイトメトリーアッセイ
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
A DNA/Ki67-Based Flow Cytometry Assay for Cell Cycle Analysis of Antigen-Specific CD8 T Cells in Vaccinated Mice
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ワクチン接種マウスにおける抗原特異的CD8 T細胞の細胞周期解析のためのDNA/Ki67ベースのフローサイトメトリーアッセイ

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09:17 min

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January 05, 2021

DOI:

10.3791/61867-v

DOI:
10.3791/61867-v

•

09:17 min
January 05, 2021

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1Institute of Molecular Biology and Pathology,National Research Council of Italy (CNR), 2Department of Molecular Medicine,University of Rome “Sapienza”, 3Center for Life Nano Science,Istituto Italiano di Tecnologia, 4Department of Molecular Medicine and Medical Biotechnology,University of Naples Federico II, 5Reithera Srl, 6IRCCS, Neuromed

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DNAKi67Flow CytometryCell CycleCD8 T CellsVaccinationImmune DynamicsPermeabilization BufferHoechstAntigen-Specific

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Simonetti, S., Natalini, A., Peruzzi, G., Nicosia, A., Folgori, A., Capone, S., Santoni, A., Di Rosa, F. A DNA/Ki67-Based Flow Cytometry Assay for Cell Cycle Analysis of Antigen-Specific CD8 T Cells in Vaccinated Mice. J. Vis. Exp. (167), e61867, doi:10.3791/61867 (2021).

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