Journal
/
Immunologie et infection
/
一种基于DNA/Ki67的流式细胞术测定法，用于接种疫苗的小鼠抗原特异性CD8 T细胞的细胞周期分析
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
A DNA/Ki67-Based Flow Cytometry Assay for Cell Cycle Analysis of Antigen-Specific CD8 T Cells in Vaccinated Mice
Please note that all translations are automatically generated. Click here for the English version.

一种基于DNA/Ki67的流式细胞术测定法，用于接种疫苗的小鼠抗原特异性CD8 T细胞的细胞周期分析

6,123 Views

•

09:17 min

•

January 05, 2021

DOI:

10.3791/61867-v

DOI:
10.3791/61867-v

•

09:17 min
January 05, 2021

•

9 Views
, , , , , , ,
1Institute of Molecular Biology and Pathology,National Research Council of Italy (CNR), 2Department of Molecular Medicine,University of Rome “Sapienza”, 3Center for Life Nano Science,Istituto Italiano di Tecnologia, 4Department of Molecular Medicine and Medical Biotechnology,University of Naples Federico II, 5Reithera Srl, 6IRCCS, Neuromed

Tags

DNAKi67Flow CytometryCell CycleCD8 T CellsVaccinationImmune DynamicsPermeabilization BufferHoechstAntigen-Specific

Read Article

Cite this Article

Simonetti, S., Natalini, A., Peruzzi, G., Nicosia, A., Folgori, A., Capone, S., Santoni, A., Di Rosa, F. A DNA/Ki67-Based Flow Cytometry Assay for Cell Cycle Analysis of Antigen-Specific CD8 T Cells in Vaccinated Mice. J. Vis. Exp. (167), e61867, doi:10.3791/61867 (2021).

Download .ris file

Copy

Share Video

.

Copy