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通过聚合-去聚合周期，通过有限来源的受控后翻译修改和异种型对图布林进行净化

Journal JoVE
Biochimie

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Journal JoVE Biochimie

Purification of Tubulin with Controlled Posttranslational Modifications and Isotypes from Limited Sources by Polymerization-Depolymerization Cycles

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通过聚合-去聚合周期，通过有限来源的受控后翻译修改和异种型对图布林进行净化

4,841 Views

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07:54 min

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November 05, 2020

DOI:

10.3791/61826-v

DOI:

10.3791/61826-v

•

07:54 min

November 05, 2020

•

10 Views

Satish Bodakuntla*^1,2, A.S. Jijumon*^1,2, Carsten Janke^1,2, Maria M. Magiera^1,2

¹PSL Research University, CNRS UMR3348,Institut Curie, ²Université Paris-Saclay, CNRS UMR3348,Université Paris Sud

Summary

本协议使用聚合和去聚合周期描述从中小型来源（如培养细胞或单个小鼠大脑）提纯的图布林。纯化的图布林富含特定的同位素或具有特定的翻译后修饰，可用于体外重组测定，以研究微管动力学和相互作用。

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