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体外楔形切片准备模拟在Vivvo神经元电路连接
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Journal JoVE Neurosciences
In Vitro Wedge Slice Preparation for Mimicking In Vivo Neuronal Circuit Connectivity
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体外楔形切片准备模拟在Vivvo神经元电路连接

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10:31 min

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August 18, 2020

DOI:

10.3791/61664-v

DOI:
10.3791/61664-v

•

10:31 min
August 18, 2020

•

2 Views
,
1Section on Neuronal Circuitry, National Institute on Deafness and Other Communication Disorders,NIH

Summary

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将不同的突触输入与神经元的整合最好用一种制备来测量，这种制备保留了所有突触前核，以便自然计时和电路可塑性，但大脑切片通常切断许多连接。我们开发了一个经过修改的大脑切片来模拟体内回路活动，同时保持体外实验能力。

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Cite this Article

Fischl, M. J., Weisz, C. J. C. In Vitro Wedge Slice Preparation for Mimicking In Vivo Neuronal Circuit Connectivity. J. Vis. Exp. (162), e61664, doi:10.3791/61664 (2020).

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