Journal
/
Biologie
/
En enkelt celle dissociation tilgang til molekylær analyse af urinblæren i musen efter rygmarvsskade
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury

En enkelt celle dissociation tilgang til molekylær analyse af urinblæren i musen efter rygmarvsskade

DOI:
10.3791/61455-v

•

09:50 min

•

June 17, 2020

•

, , , , , , , ,
1Department of Urology,Boston Children’s Hospital, 2Department of Surgery,Harvard Medical School, 3Division of Hematology/Oncology,Harvard Medical School Boston, 4Dana-Farber Cancer Institute, 5Broad Institute, 6Biological Biomedical Sciences Program, Division of Medical Sciences,Harvard Medical School, 7Vascular Biology Program,Boston Children’s Hospital

Chapitres

  • 00:04Introduction
  • 00:57Mouse Preparation
  • 01:48Spinal Cord Injury (SCI)
  • 03:17Tissue Perfusion and Procurement
  • 04:34Cell Dissociation and Single Cell Suspension Preparation
  • 05:59Cell Staining
  • 06:44Flow Cytometry Analysis
  • 07:43Results: Representative Mouse Bladder Collagen Expression and Immune Cell Population Analyses
  • 08:54Conclusion

Summary

Traduction automatique

Traduction automatique

Målet med denne protokol er at anvende en optimeret væv dissociation protokol til en mus model af rygmarvsskader og validere tilgangen til enkelt celle analyse ved flow cytometry.

Tags

Single Cell DissociationMolecular AnalysisUrinary BladderMouseSpinal Cord InjuryFACS AnalysisLaminectomySpinal Cord TransectionBladder Expression

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Spinal Cord Electrophysiology

Single-celle Mikroinjektion for Cell Kommunikation Analyse

Analyse af Global RNA-syntese på enkelt celle niveau efter Hypoxia

Open source Single-partikel Analyse for Super-resolution Microscopy med VirusMapper

Cystometric og ekstern urethrae Sphincter målinger i vågen rotter med indopereret kateter og elektroder giver mulighed for gentagne målinger

Encellede mikrofluid analyse af Bacillus subtilis

Nye metode af Plasmid DNA levering til musen blære Urothelium af elektroporation

Analyse af Beta-celle funktion ved hjælp af encellede opløsning Calcium Imaging i zebrafisk Holme

Kerneisolering fra voksen musenyre til RNA-sekventering med en kerne

Rygmarvstranssektion i Xenopus laevis tadpoles

Read Article