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Recherche en cancérologie
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Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
Journal JoVE
Recherche en cancérologie
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Journal JoVE Recherche en cancérologie
Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

DOI:
10.3791/61355-v

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07:50 min

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September 18, 2020

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, , , , , ,
1Department of Surgery, The Sidney Kimmel Comprehensive Cancer Center,Johns Hopkins University School of Medicine, 2Department of Coloproctological Surgery,Juntendo University Faculty of Medicine, 3Department of Surgery,Juntendo University Shizuoka Hospital

Chapitres

  • 00:04Introduction
  • 00:49Washing the Slides
  • 01:51Scraping the Slides
  • 03:01Bisulfite Treatment
  • 05:32Results: Genome-Wide DNA Methylation Analysis in Gastric Cancer Patients
  • 07:24Conclusion

Summary

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Traduction automatique

Herein, we describe a procedure for genome-wide analysis of DNA methylation in gastrointestinal cancers. The procedure is of relevance to studies that investigate relationships between methylation patterns of genes and factors contributing to carcinogenesis in gastrointestinal cancers.

Tags

DNA MethylationGenome-wide AnalysisGastrointestinal CancerEpigenetic BiomarkersLA PlatformComplex AberrationFormalin-fixed Paraffin EmbeddedMacrodissectionBisulfite Conversion

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