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In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
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Génétique
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Journal JoVE Génétique
In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

6,852 Views

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09:16 min

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March 25, 2020

DOI:

10.3791/60807-v

DOI:
10.3791/60807-v

•

09:16 min
March 25, 2020

•

1 Views
,
1International Institute of Molecular and Cell Biology, Warsaw (IIMCB)

Tags

In Vitro Directed EvolutionRestriction EndonucleaseSequence SpecificityIn Vitro CompartmentalizationDirected EvolutionSubsaturation MutagenesisOligonucleotide SynthesisOil Surfactant MixtureLibrary Preparation

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Cite this Article

Skowronek, K. J., Bochtler, M. In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity. J. Vis. Exp. (157), e60807, doi:10.3791/60807 (2020).

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