Journal
/
/
siRNA electroporation til modulere Autofagi i herpes simplex virus type 1-infiserte monocytt-avledet dendrittiske celler
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells
DOI:

09:10 min

October 28, 2019

, , , , , ,

Chapitres

  • 00:04Titre
  • 01:02Generation and Handling of Immature Dendritic Cells and Mature Dendritic Cells
  • 02:02Infection Procedure of DCs with HSV-1 and Interference of HSV-1-induced Autophagic Flux via Spautin-1 or Bafilomycin-A1
  • 04:17Interference of HSV-1-induced Autophagic Flux via Electroporation of iDCs Using FIP200-siRNA
  • 06:21Results: Analyses of Autophagic Flux in HSV-1-infected iDCs and mDCs
  • 08:10Conclusion

Summary

Traduction automatique

I denne studien, presenterer vi hemmer-og siRNA-baserte strategier for å forstyrre autophagic Flux i herpes simplex virus type-1 (HSV-1)-infiserte monocytt-avledet dendrittiske celler.

Vidéos Connexes

Read Article