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Lentiviral CRISPR/Cas9介导基因组编辑，用于疾病模型中造血细胞的研究
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Immunologie et infection
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Journal JoVE Immunologie et infection
Lentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models
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Lentiviral CRISPR/Cas9介导基因组编辑，用于疾病模型中造血细胞的研究

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08:14 min

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October 03, 2019

DOI:

10.3791/59977-v

DOI:
10.3791/59977-v

•

08:14 min
October 03, 2019

•

17 Views
, , , , , , , ,
1Hematovascular Biology Center, Robert M. Berne Cardiovascular Research Center,University of Virginia School of Medicine

Summary

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本文介绍了CRISPR/Cas9系统对鼠血干细胞和祖细胞（HSPC）进行高效基因组编辑的协议，以快速开发具有造血系统特异性基因修饰的小鼠模型系统。

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Cite this Article

Sano, S., Wang, Y., Evans, M. A., Yura, Y., Sano, M., Ogawa, H., Horitani, K., Doviak, H., Walsh, K. Lentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models. J. Vis. Exp. (152), e59977, doi:10.3791/59977 (2019).

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