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Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes

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Génétique

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Journal JoVE Génétique

Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes

Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes

7,803 Views

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09:10 min

•

July 09, 2019

DOI:

10.3791/59850-v

DOI:

10.3791/59850-v

•

09:10 min

July 09, 2019

•

7 Views

Wilson Pak-Kin Lou*^1,2, Liqun Wang*³, Cheng Long, Lei Liu, Ji-Feng Fei

¹School of Life Sciences,South China Normal University, ²The Research Institute of Molecular Pathology (IMP),Vienna Biocenter (VBC), ³Institute for Brain Research and Rehabilitation (IBRR),South China Normal University

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