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Separação de Epiderme de Rato e Derme com Termolise para Detectar mRNA inflamatório específico do local e proteína
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Immunologie et infection
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Journal JoVE Immunologie et infection
Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein

Separação de Epiderme de Rato e Derme com Termolise para Detectar mRNA inflamatório específico do local e proteína

4,906 Views

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08:45 min

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September 29, 2021

DOI:

10.3791/59708-v

DOI:
10.3791/59708-v

•

08:45 min
September 29, 2021

•

2 Views
, , , , ,
1Department of Anatomy and Cell Biology,Oklahoma State University Center for Health Sciences, 2Institute for Shock Physics,Washington State University

Tags

EpidermisDermisThermolysinSkin InflammationInflammatory MediatorsNeurotrophinsWound HealingSkin DiseaseChronic WoundsBurnsEpithelial SurfacesGI TractCorneaSprague Dawley RatLambda CarrageenanEdemaGlabrous Hind PawMicrodissection ForcepsDMEMStratum Corneum

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Cite this Article

Gujar, V., Anderson, M. B., Miller, K. E., Pande, R. D., Nawani, P., Das, S. Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein. J. Vis. Exp. (175), e59708, doi:10.3791/59708 (2021).

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