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ラット表皮と真皮をサーモリジンと分離して、部位特異的炎症性mRNAおよびタンパク質を検出する
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Immunologie et infection
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Journal JoVE Immunologie et infection
Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein
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ラット表皮と真皮をサーモリジンと分離して、部位特異的炎症性mRNAおよびタンパク質を検出する

4,906 Views

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08:45 min

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September 29, 2021

DOI:

10.3791/59708-v

DOI:
10.3791/59708-v

•

08:45 min
September 29, 2021

•

2 Views
, , , , ,
1Department of Anatomy and Cell Biology,Oklahoma State University Center for Health Sciences, 2Institute for Shock Physics,Washington State University

Tags

EpidermisDermisThermolysinSkin InflammationInflammatory MediatorsNeurotrophinsWound HealingSkin DiseaseChronic WoundsBurnsEpithelial SurfacesGI TractCorneaSprague Dawley RatLambda CarrageenanEdemaGlabrous Hind PawMicrodissection ForcepsDMEMStratum Corneum

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Gujar, V., Anderson, M. B., Miller, K. E., Pande, R. D., Nawani, P., Das, S. Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein. J. Vis. Exp. (175), e59708, doi:10.3791/59708 (2021).

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