This content is Free Access.
1Biological Design Center,Boston University, 2Department of Biomedical Engineering,Boston University, 3Program in Molecular Biology, Cell Biology and Biochemistry,Boston University, 4Department of Bioengineering,Rice University, 5Wyss Institute for Biologically Inspired Engineering,Harvard University
Chapitres
- 00:04Titre
- 00:58Sterilizing the System
- 02:56Loading the Culture Vials
- 03:51Configuring eVOLVER Software and Programming Algorithmic Culture Routines
- 04:48Inoculating and Monitoring Experiment in Real-time
- 05:47Results: Fitness Surface Construction
- 06:35Conclusion
EVOLVER Framework muliggør kontinuerlig mikrobiel kultur med høj opløsning og dynamisk kontrol over eksperimentelle parametre. Denne protokol demonstrerer, hvordan systemet kan anvendes til at gennemføre et komplekst fitness eksperiment, der vejleder brugerne om programmering af automatiseret kontrol over mange individuelle kulturer, måling, indsamling og interaktion med eksperimentelle data i realtid.
