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Designing Automated, High-throughput, Continuous Cell Growth Experiments Using eVOLVER
Journal JoVE
Génétique
This content is Free Access.
Journal JoVE Génétique
Designing Automated, High-throughput, Continuous Cell Growth Experiments Using eVOLVER
DOI:

07:26 min

May 19, 2019

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Chapitres

  • 00:04Titre
  • 00:58Sterilizing the System
  • 02:56Loading the Culture Vials
  • 03:51Configuring eVOLVER Software and Programming Algorithmic Culture Routines
  • 04:48Inoculating and Monitoring Experiment in Real-time
  • 05:47Results: Fitness Surface Construction
  • 06:35Conclusion

Summary

Traduction automatique

The eVOLVER framework enables high-throughput continuous microbial culture with high resolution and dynamic control over experimental parameters. This protocol demonstrates how to apply the system to conduct a complex fitness experiment, guiding users on programming automated control over many individual cultures, measuring, collecting, and interacting with experimental data in real-time.

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