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评估介入疗法对人体粪便微生物群影响的体内批处理培养模型
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Immunologie et infection
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Journal JoVE Immunologie et infection
An In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota
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评估介入疗法对人体粪便微生物群影响的体内批处理培养模型

DOI:
10.3791/59524-v

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07:15 min

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July 31, 2019

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, , , , , ,
1Department of Internal Medicine- Molecular Medicine,Wake Forest School of Medicine, 2Department of Microbiology and Immunology,Wake Forest School of Medicine, 3Department of Food Science and Technology,Isfahan University of Technology, 4Research Institute for Biotechnology and Bioengineering, College of Agriculture,Isfahan University of Technology, 5Department of Geriatrics and Gerontology,Wake Forest School of Medicine

Chapitres

  • 00:04Titre
  • 01:42Preparation for Fermentation Experiment
  • 03:52Fermentation and Sampling
  • 05:01Results: The Effect of Prebiotic Treatment
  • 06:28Conclusion

Summary

Traduction automatique

Traduction automatique

该协议描述了人类粪便微生物群的体外批量培养发酵系统,使用硫素(一种众所周知的益生菌和研究最广泛的微生物群调制器之一)来证明该系统在估计特定效果时的使用粪便微生物群成分和代谢活动的干预措施。

Tags

Gut MicrobiomeIn Vitro Batch-culture ModelFecal MicrobiotaMicrobiome ModulatorsProbioticsPrebioticsDietsDrugsShort-chain Fatty AcidsAnaerobic ConditionsAseptic ConditionsFermentation MediaAnaerobic Dilution Solution

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