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Biochemistry

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JoVE Journal Biochemistry
Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells
 

Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells

Article DOI: 10.3791/59519-v • 13:04 min • May 16th, 2019
May 16th, 2019 •
*1, *2, 3, 4, 2, 1
1Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 2Department of Chemistry, Skidmore College, 3Candiac MR Center, Beth Israel Deaconess Medical Center, 4Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero
* These authors contributed equally

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résumé
Automatic Translation

Cultured primary or established cell lines are commonly used to address fundamental biological and mechanistic questions as an initial approach before using animal models. This protocol describes how to prepare whole cell extracts and subcellular fractions for studies of zinc (Zn) and other trace elements with atomic absorbance spectroscopy.

Tags

Atomic Absorbance Spectroscopy Intracellular Zinc Pools Transition Metals Metal Homeostasis Cellular Destinations Of Metals Analytical Techniques Graphite Furnace Atomic Absorption Spectroscopy GF-AAS Trace Zinc Analysis Low Volume Samples Metal Misbalances Pathological Conditions Anemia Acrodermatitis Enteropathica Wilson's And Menkes' Diseases
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