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非還元 SDS − PAGE 分析と化学的架橋を組み合わせて、培養中の哺乳動物細胞におけるジスルフィド結合によって安定化された多量体複合体を検出する
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Chapitres
- 00:04Titre
- 00:34Harvesting Cells After Blocking Free Cysteine Residues Using Iodoacetamide
- 02:02Extraction of Protein and Sample Preparation
- 03:13In Vivo Formaldehyde Cross-linking of Endogenous Proteins in U-2 OS Cells
- 04:30Fractionation of Nuclei
- 06:11Extraction of Protein and Sample Preparation
- 06:51SDS-PAGE Analysis
- 07:53Results: Analyzing Multimeric Complexes Using a Two-step Method of Non-reducing SDS-PAGE Analysis and Formaldehyde Cross-linking
- 09:04Conclusion
