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레브 면역 침전 및 질량 분광법을 통한 HIV-1 복제 중 핵균 요인 식별
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Immunologie et infection
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Journal JoVE Immunologie et infection
Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry
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레브 면역 침전 및 질량 분광법을 통한 HIV-1 복제 중 핵균 요인 식별

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09:38 min

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June 26, 2019

DOI:

10.3791/59329-v

DOI:
10.3791/59329-v

•

09:38 min
June 26, 2019

•

7 Views
, , , , , ,
1Molecular and Cellular Biology Department,Beckman Research Institute at the City of Hope, 2Irell & Manella Graduate School of Biological Sciences, 3Department of Molecular Immunology,Beckman Research Institute and the City of Hope

Tags

HIV-1Nucleolar FactorsRev ImmunoprecipitationMass SpectrometryViral FactorsHost FactorsInfectious CycleProtein CharacterizationHeLa CellsPlasmid TransfectionCell LysisProtease InhibitorImmunoprecipitation

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Arizala, J. A. C., Chomchan, P., Li, H., Moore, R., Ge, H., Ouellet, D. L., Rossi, J. J. Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry. J. Vis. Exp. (148), e59329, doi:10.3791/59329 (2019).

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