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Measuring Enzymatic Stability by Isothermal Titration Calorimetry
 

Measuring Enzymatic Stability by Isothermal Titration Calorimetry

Article DOI: 10.3791/59302-v • 08:37 min • March 26th, 2019
March 26th, 2019 •
1, 1, 2, 1
1Department of Nutrition, Dietetics and Food Science, Brigham Young University, 2Department of Chemistry and Biochemistry, Brigham Young University

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résumé
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The thermal stability of enzyme activity is readily measured by isothermal titration calorimetry (ITC). Most protein stability assays currently used measure protein unfolding, but do not provide information about enzymatic activity. ITC enables direct determination of the effect of enzyme modifications on the stability of enzyme activity.

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Isothermal Titration Calorimetry Enzymatic Stability Enzyme Activity Enzyme Modifications Man Hours Traditional Enzyme Stability Assays Sodium Acetate Buffer PH Measurement Hydrochloric Acid Sodium Hydroxide Enzyme Solution
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