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환경 샘플에서 높은 처리량 Siderophore 심사: 식물 조직, 대량 토양, 및 Rhizosphere 토양
Journal JoVE
Environnement
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Journal JoVE Environnement
High-throughput Siderophore Screening from Environmental Samples: Plant Tissues, Bulk Soils, and Rhizosphere Soils
DOI:

12:36 min

February 09, 2019

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Chapitres

  • 00:04Titre
  • 01:01Preparation of Chrome Azurol S (CAS)-Fe-Agar Medium
  • 03:35Pyoverdine/EDTA Standard Preparation
  • 05:04Collection of Environmental Samples: Soil and Plant Tissues
  • 06:38Preparation of Siderophore Enrichment Cultures and CAS-Fe Siderophore Production Assay
  • 08:11CAS-Fe Agar Assays for Detection of Siderophore Production in Environmental Samples
  • 09:34Results: Pyoverdine Equivalents of Siderophore Enrichment Cultures
  • 11:28Conclusion

Summary

Traduction automatique

우리는 지구 시스템에서 미 량 영양소 bioavailability 및 매출에 기여 하는 siderophore 잠재적인 환경 샘플의 신속한 심사에 대 한 프로토콜을 제시.

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