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Engenharia genética de células de Dictyostelium discoideum com base na seleção e crescimento de bactérias

Journal JoVE
Génétique

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Journal JoVE Génétique

Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria

Engenharia genética de células de Dictyostelium discoideum com base na seleção e crescimento de bactérias

12,280 Views

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06:08 min

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January 25, 2019

DOI:

10.3791/58981-v

DOI:

10.3791/58981-v

•

06:08 min

January 25, 2019

•

6 Views

Peggy Paschke, David A. Knecht, Thomas D. Williams, Peter A. Thomason, Robert H. Insall, Jonathan R. Chubb⁵, Robert R. Kay, Douwe M. Veltman

¹MRC Laboratory of Molecular Biology, ²Department of Molecular and Cell Biology,University of Connecticut, ³Cancer Research UK Beatson Institute Glasgow, ⁴MRC Laboratory for Molecular Cell Biology,University College London, ⁵Department of Cell and Developmental Biology,University College London

Tags

Dictyostelium Discoideum Genetic Engineering Bacterial Selection Growth On Bacteria Exochromosomal Plasmid Transfection Microscopy Klebsiella Aerogenes Sorensen Buffer LB Medium Optical Density H40 Electroporation Buffer SM Medium Agar Plate Cell Transfer Centrifugation DNA Transformation

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