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January 10, 2019
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1Hotchkiss Brain Institute,University of Calgary, 2Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine,University of Calgary, 3Alberta Children’s Hospital Research Institute,University of Calgary, 4Department of Clinical Neurosciences, Cumming School of Medicine,University of Calgary
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该协议描述了为基于液滴、高通量的单细胞 rna-seq 制剂准备健康的成年哺乳动物单细胞所需的一般过程和质量控制检查。还提供了测序参数、读取对齐和下游单细胞生物信息学分析。
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Stratton, J. A., Sinha, S., Shin, W., Labit, E., Chu, T., Shah, P. T., Midha, R., Biernaskie, J. Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues. J. Vis. Exp. (143), e58709, doi:10.3791/58709 (2019).
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