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Immunologie et infection
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Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

DOI:
10.3791/58407-v

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08:36 min

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November 01, 2018

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, , , , ,
1Department of Microbiology and Infection Control,Osaka Medical College

Chapitres

  • 00:04Titre
  • 01:06Synthesis of the DENV 3’UTR RNA Standard
  • 03:15Processing of Virus Samples for RT-qPCR
  • 04:19Real-time PCR Analysis
  • 06:01Results: Real-time Quantitative Polymerase Chain Reaction of Virus RNA
  • 07:58Conclusion

Summary

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Real-time quantitative polymerase chain reaction analysis combined with reverse transcription (RT-qPCR) has been widely used to measure the level of RNA virus infections. Here we present a direct RT-qPCR assay, which does not require an RNA purification step, developed for the quantification of several RNA viruses, including dengue virus.

Tags

Dengue VirusRNA QuantificationReal-time PCRRNA StandardIn Vitro TranscriptionRNA PurificationViral RNA DetectionVirus ResearchDrug ScreeningClinical Diagnosis

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