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Utvärdering av Zika Virus-specifika T-cell svar i Immunoprivileged organ av infekterade Ifnar1- / - möss
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1School of Laboratory Medicine and Life Sciences,Wenzhou Medical University, 2NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention, 3State Key Laboratory of Agrobiotechnology, College of Biological Sciences,China Agricultural University, 4Research Network of Immunity and Health (RNIH), Beijing Institutes of Life Science,Chinese Academy of Sciences, 5Laboratory Animal Center,Chinese Center for Disease Control and Prevention, 6CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology,Chinese Academy of Sciences
Chapitres
- 00:04Titre
- 01:15Zika Virus (ZIKV) Infection, Immunization, and Splenic Monocyte Isolation
- 03:19Brain and Testicular Monocyte Isolation
- 06:10Flow Cytometry
- 07:26Results: ZIKV Infection Characterization in Ifnar1-/- Mice
- 09:10Conclusion
Ett protokoll för att utvärdera antigen-specifika T-cell svar i immunoprivileged organ Ifnar1- / - murina modellen för Zika virus (ZIKV) infektionen beskrivs. Denna metod är avgörande för att utreda de cellulära mekanismerna för skydd och immunpatogenes ZIKV vacciner och är också värdefull för deras effekt utvärdering.
Tags
Zika VirusT-cell ResponsesImmunoprivileged OrgansIfnar1-/- MiceTetramer StainingImmunopathogenesisGuillain-Barre SyndromeTestes InjuryInterferon Alpha/beta Receptor KnockoutBrainTestesSpleenFocus-forming UnitsRetro-orbital InoculationAbdominal SurgerySingle-cell SuspensionRed Blood Cell LysisFlow Cytometry
