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A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates
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Génétique
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Journal JoVE Génétique
A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates

A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates

6,437 Views

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11:49 min

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August 21, 2018

DOI:

10.3791/57816-v

DOI:
10.3791/57816-v

•

11:49 min
August 21, 2018

•

1 Views
1Department of Molecular, Cellular and Developmental Biology,University of Colorado at Boulder

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Saturation MutagenesisPhosphorothioatesRNAProtein Binding SiteIn Vitro TranscriptionT7 RNA Polymerase5′ End LabelingAlpha-thio NTPAlkaline PhosphataseT4 Polynucleotide KinaseGene Regulation

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Singh, R. A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates. J. Vis. Exp. (138), e57816, doi:10.3791/57816 (2018).

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