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泡沫病毒 Intasomes 原型的组装与纯化
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Immunologie et infection
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Journal JoVE Immunologie et infection
Assembly and Purification of Prototype Foamy Virus Intasomes
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泡沫病毒 Intasomes 原型的组装与纯化

5,347 Views

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10:20 min

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March 19, 2018

DOI:

10.3791/57453-v

DOI:
10.3791/57453-v

•

10:20 min
March 19, 2018

•

2 Views
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1Department of Cancer Biology and Genetics,The Ohio State University College of Medicine

Summary

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重组逆转录病毒整合酶和 dna 寡聚物模仿病毒 dna 末端可以形成一个酶活跃的复杂称为 intasome。Intasomes 可用于生物化学、结构和动力学研究。本协议详细介绍了如何组装和纯化原型泡沫病毒 intasomes。

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Cite this Article

Mackler, R. M., Lopez Jr., M. A., Yoder, K. E. Assembly and Purification of Prototype Foamy Virus Intasomes. J. Vis. Exp. (133), e57453, doi:10.3791/57453 (2018).

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