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Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

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Biochimie

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Journal JoVE Biochimie

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

DOI:

10.3791/57293-v

•

08:53 min

•

May 07, 2018

•

Kotaro Hama, Yuko Fujiwara, Kazuaki Yokoyama

¹Faculty of Pharmaceutical Sciences,Teikyo University

Chapitres

00:05Titre
00:33Preparation of Lipid Samples
03:06Sample Preparation for Calibration Curve and Method Validation
03:50SM Analysis by LC-ESI-MS/MS
05:33Batch File Creation and Assigning the MS Three Product Ion Spectra of Sphingomyelin
06:23Quantitative Analysis of SM by LC-ESI-MS/MS
07:02Results: Validating the Quantitative and Qualitative Method for Sphingomyelin
08:01Conclusion

Summary

Traduction automatique

Here, we present a protocol to quantify and qualify each sphingomyelin species using multiple reaction monitoring and MS/MS/MS mode, respectively.

Tags

Keywords: Quantitative Analysis Qualitative Analysis Sphingomyelin LC-MS Stable Isotopically Labeled Lipid Biology Lipid Characterization HeLa Cells Cell Culture Extraction Chromatography Mass Spectrometry Internal Standard Organic Solvents

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