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CRISPR/Cas9 对小鼠和人造血祖细胞的高效基因破坏

Journal JoVE
Génétique

This content is Free Access.

Journal JoVE Génétique

Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9

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CRISPR/Cas9 对小鼠和人造血祖细胞的高效基因破坏

12,955 Views

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08:27 min

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April 10, 2018

DOI:

10.3791/57278-v

DOI:

10.3791/57278-v

•

08:27 min

April 10, 2018

•

6 Views

Lorenzo Brunetti*^1,2,3, Michael C. Gundry*^1,2,4, Ayumi Kitano⁴, Daisuke Nakada^1,2,4, Margaret A. Goodell^1,2,4,5

¹Stem Cells & Regenerative Medicine Center,Baylor College of Medicine, ²Center for Cell and Gene Therapy,Baylor College of Medicine, ³Centro di Ricerca Emato-Oncologica (CREO),University of Perugia, ⁴Department of Molecular & Human Genetics,Baylor College of Medicine, ⁵Texas Children’s Hospital & Houston Methodist Hospital

Summary

本文介绍了一种快速 CRISPR/Cas9-mediated 基因在小鼠和人原发性造血细胞中的破坏的协议。Cas9-sgRNA ribonucleoproteins 是通过电穿孔与 sgRNAs 产生通过体外转录和商业 Cas9。在有限的时间和财务成本下实现了高编辑效率。

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