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Stereotaxic Surgery for Genetic Manipulation in Striatal Cells of Neonatal Mouse Brains
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Neurosciences
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Journal JoVE Neurosciences
Stereotaxic Surgery for Genetic Manipulation in Striatal Cells of Neonatal Mouse Brains

Stereotaxic Surgery for Genetic Manipulation in Striatal Cells of Neonatal Mouse Brains

DOI:
10.3791/57270-v

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09:44 min

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July 10, 2018

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, ,
1Institute of Neuroscience,National Yang-Ming University, 2Brain Research Center,National Yang-Ming University

Chapitres

  • 00:04Titre
  • 00:40Preparing the Microinjection Apparatus
  • 04:09Microinjecting Neonatal Mice
  • 07:30Results: Analysis of Injection of Cre-expressing Virus into P2 Striatum
  • 09:03Conclusion

Summary

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We describe a protocol of stereotaxic surgery with a homemade head-fixed device for microinjecting reagents into the striatum of neonatal mouse brains. This technique allows genetic manipulation in neuronal cells of specific regions of neonatal mouse brains.

Tags

Stereotaxic SurgeryGenetic ManipulationStriatal CellsNeonatal Mouse BrainsPostnatal DevelopmentVisual DemonstrationHome-made DeviceTargeted InjectionsHead TrayStainless Steel NeedlesChloroformEthanolMicroinjection AssemblyPE20 Polyethylene TubingPE10 Tubing26 Gauge Syringe30 Gauge Injection Needle10 Microliter Microinjection Syringe25 Gauge Syringe0.1% Fast Green DyeFiltered Solution

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