Journal
/
Neurosciences
/
Quantitative Analysis of Neuronal Dendritic Arborization Complexity in Drosophila
Journal JoVE
Neurosciences
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Neurosciences
Quantitative Analysis of Neuronal Dendritic Arborization Complexity in Drosophila

Quantitative Analysis of Neuronal Dendritic Arborization Complexity in Drosophila

DOI:
10.3791/57139-v

•

07:13 min

•

January 07, 2019

•

, ,
1Genetics and Aging Research Unit, Department of Neurology, Massachusetts General Hospital,Mass General Institute of Neurodegenerative Disease, 2Department of Geriatric Neurology, Nanlou Clinical Division,PLA General Hospital

Chapitres

  • 00:04Titre
  • 01:01Larvae Collection and Dissection
  • 02:57Imaging Processing
  • 05:32Results: Silencing Sox102F Affects Dendrite Size
  • 06:23Conclusion

Summary

Traduction automatique

Traduction automatique

This protocol focuses on quantitative analysis of neuronal dendritic arborization complexity (NDAC) in Drosophila, which can be used for studies of dendritic morphogenesis.

Tags

Dendritic ArborizationDrosophilaSOX5 GeneNeuro-dendritesMorphogenesisNeurodegenerative DiseaseUAS-GFPPpk-GAL4UAS-Sox102F-RNAiThird Instar LarvaeDissectionPBSPFAAntifade Mounting MediumConfocal MicroscopyFiji ImageJDendrite Length

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Automated Sholl Analysis of Digitized Neuronal Morphology at Multiple Scales

Analysis of Dendritic Spine Morphology in Cultured CNS Neurons

The Analysis of Purkinje Cell Dendritic Morphology in Organotypic Slice Cultures

Optogenetic Perturbation of Neural Activity with Laser Illumination in Semi-intact Drosophila Larvae in Motion

Fast Micro-iontophoresis of Glutamate and GABA: A Useful Tool to Investigate Synaptic Integration

Assessment of Dendritic Arborization in the Dentate Gyrus of the Hippocampal Region in Mice

Quantitative Analysis of Climbing Defects in a Drosophila Model of Neurodegenerative Disorders

Analyzing Dendritic Morphology in Columns and Layers

Assessment of Hippocampal Dendritic Complexity in Aged Mice Using the Golgi-Cox Method

Quantitative Cell Biology of Neurodegeneration in Drosophila Through Unbiased Analysis of Fluorescently Tagged Proteins Using ImageJ

Read Article