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Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
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Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

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14:02 min

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April 09, 2018

DOI:

10.3791/57069-v

DOI:
10.3791/57069-v

•

14:02 min
April 09, 2018

•

1 Views
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1Institute of Biochemistry, Faculty of Life Sciences,University of Leipzig

Tags

GPCRPhoto-crosslinkingBioorthogonal ChemistryNon-canonical Amino AcidsAmber Codon SuppressionFluorescence AssayMammalian CellsProtein-protein InteractionsLigand BindingLive Cell Imaging

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Serfling, R., Seidel, L., Böttke, T., Coin, I. Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells. J. Vis. Exp. (134), e57069, doi:10.3791/57069 (2018).

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