A method of constructing a phylogenetic tree based on sequence homology of SWEETs from eukaryotes and SemiSWEETs from prokaryotes is described. Phylogenetic analysis is a useful tool for explaining the evolutionary relatedness between homologous proteins or genes from different organism groups.
Zhang, D., Kan, X., Huss, S. E., Jiang, L., Chen, L., Hu, Y. Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin. J. Vis. Exp. (138), e56684, doi:10.3791/56684 (2018).