Journal
/
/
Caracterización estructural de polisacáridos de pared celular mananos en plantas con ritmo
Journal JoVE
Biochimie
This content is Free Access.
Journal JoVE Biochimie
Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
DOI:

11:06 min

October 16, 2017

, ,

Chapitres

  • 00:05Titre
  • 00:37Aliquoting and Pre-treatment of Alcohol Insoluble Residue (AIR)
  • 02:11Hydrolysis of AIR with Glycosyl Hydrolases
  • 03:20Derivitization of Oligosaccharides
  • 04:29Preparation of PACE Gels
  • 06:33Running a PACE Gel
  • 07:51Visualizing a PACE Gel
  • 09:07Results: Characterization of Plant Cell Wall Glucomannam Structure
  • 10:30Conclusion

Summary

Traduction automatique

Un protocolo para el análisis estructural de polisacáridos por electroforesis del gel (PACE), usando Manano como ejemplo, se describe.

Vidéos Connexes

Read Article