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DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

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Biochimie

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Journal JoVE Biochimie

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

13,900 Views

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07:38 min

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October 06, 2017

DOI:

10.3791/56228-v

DOI:

10.3791/56228-v

•

07:38 min

October 06, 2017

•

9 Views

Eliza L. Lewis, Aaron M. Leconte

¹Department of Chemistry,W.M. Keck Science Department of Claremont Mckenna, Pitzer, and Scripps College

Tags

DNA Polymerase Activity Assay Near-infrared Fluorescent Acrylamide Gel Electrophoresis DNA Synthesis DNA Modifications Temperature Control Buffer Preparation Quenching Reaction Acrylamide Gel Casting Gel Plate Preparation Spacers Gel Tape Water Level Marking

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