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低丰度胚胎标本的染色质沉淀 (芯片) 协议

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Génétique

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Journal JoVE Génétique

Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples

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低丰度胚胎标本的染色质沉淀 (芯片) 协议

15,337 Views

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12:47 min

•

August 29, 2017

DOI:

10.3791/56186-v

DOI:

10.3791/56186-v

•

12:47 min

August 29, 2017

•

15 Views

Rizwan Rehimi¹, Michaela Bartusel¹, Francesca Solinas², Janine Altmüller², Alvaro Rada-Iglesias^1,3

¹Center for Molecular Medicine Cologne (CMMC),University of Cologne, ²Cologne Center for Genomics (CCG),University of Cologne, ³Cologne Excellence Cluster for Cellular Stress Responses in Aging-Associated Diseases (CECAD), Research Center and Systems Biology of Ageing Cologne,University of Cologne

Summary

在这里, 我们描述了染色质沉淀 (芯片) 和芯片序列库的准备协议, 以产生全球表剖面从丰鸡胚胎标本。

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