This protocol describes the implementation of an asymmetric-detection time-stretch optical microscopy system for single-cell imaging in ultrafast microfluidic flow and its applications in imaging flow cytometry.
Tang, A. H. L., Lai, Q. T. K., Chung, B. M. F., Lee, K. C. M., Mok, A. T. Y., Yip, G. K., Shum, A. H. C., Wong, K. K. Y., Tsia, K. K. Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM). J. Vis. Exp. (124), e55840, doi:10.3791/55840 (2017).