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生細胞測定の嗅覚受容体活性化アッセイを用いた、リアルタイムキャンプ

Journal JoVE
Neurosciences

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Journal JoVE Neurosciences

Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay

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生細胞測定の嗅覚受容体活性化アッセイを用いた、リアルタイムキャンプ

8,683 Views

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09:11 min

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October 02, 2017

DOI:

10.3791/55831-v

DOI:

10.3791/55831-v

•

09:11 min

October 02, 2017

•

5 Views

Yuetian Zhang*¹, Yi Pan*¹, Hiroaki Matsunami^2,3, Hanyi Zhuang^1,4

¹Department of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of National Ministry of Education,Shanghai Jiao Tong University School of Medicine, ²Department of Molecular Genetics and Microbiology,Duke University Medical Center, ³Department of Neurobiology, Duke Institute for Brain Sciences,Duke University Medical Center, ⁴Institute of Health Science,Chinese Academy of Science/Shanghai Jiao Tong University School of Medicine

Summary

嗅覚受容体の機能を特徴づける deorphanization プロセスの不可欠な部分を提供しています。キャンプの試金を使用してリアルタイムの嗅覚受容体の活性化を測定する手法を提案します。

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