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实时 cAMP 检测气味受体激活的活细胞测量

Journal JoVE
Neurosciences

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Journal JoVE Neurosciences

Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay

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实时 cAMP 检测气味受体激活的活细胞测量

8,683 Views

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09:11 min

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October 02, 2017

DOI:

10.3791/55831-v

DOI:

10.3791/55831-v

•

09:11 min

October 02, 2017

•

5 Views

Yuetian Zhang*¹, Yi Pan*¹, Hiroaki Matsunami^2,3, Hanyi Zhuang^1,4

¹Department of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of National Ministry of Education,Shanghai Jiao Tong University School of Medicine, ²Department of Molecular Genetics and Microbiology,Duke University Medical Center, ³Department of Neurobiology, Duke Institute for Brain Sciences,Duke University Medical Center, ⁴Institute of Health Science,Chinese Academy of Science/Shanghai Jiao Tong University School of Medicine

Summary

表征气味受体的功能在 deorphanization 过程中起着不可缺少的作用。我们描述了一种方法来测量气味受体的激活, 在实时使用阵营分析。

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