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Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve
Journal JoVE
Biochimie
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Journal JoVE Biochimie
Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve
DOI:

09:13 min

June 14, 2017

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Chapitres

  • 00:05Titre
  • 01:17Mitral Valve Preparation
  • 03:39Protein Extraction
  • 07:20Results: Four Newly Identified Mitral Valve Proteins
  • 08:28Conclusion

Summary

Traduction automatique

The protein composition of the human mitral valve is still partially unknown, because its analysis is complicated by low cellularity and therefore by low protein biosynthesis. This work provides a protocol to efficiently extract protein for the analysis of the mitral valve proteome.

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